---
title: Researchers at The Scripps Research Institute Evaluate Demeter EVPrep™ for Compatibility with Mass Spectrometry
description: Results of a technology evaluation of the Demeter EVPrep automated EV isolation system by researchers at The Scripps Research Institute.
image: https://exokeryx.com/hubfs/Volcano%20Plot2.png
---

 Feb 16, 2026 11:42:46 AM

# Researchers at The Scripps Research Institute Evaluate Demeter EVPrep™ for Compatibility with Mass Spectrometry

![Picture of Janet Anna](https://exokeryx.com/hs-fs/hubfs/Janet%20Headshot%20(1).jpg?width=50&name=Janet%20Headshot%20(1).jpg) [ Janet Anna ](https://exokeryx.com/exoblog/author/janet-anna)

Share: [facebook-f icon ](http://www.facebook.com/share.php?u=https://exokeryx.com/exoblog/extracellular-vesicle-isolation-with-demeter-evprep-demonstrates-compatibility-with-mass-spectrometry-proteomic-analysis) [linkedin-in icon ](http://www.linkedin.com/shareArticle?mini=true&url=https://exokeryx.com/exoblog/extracellular-vesicle-isolation-with-demeter-evprep-demonstrates-compatibility-with-mass-spectrometry-proteomic-analysis) [Twitter icon ](https://twitter.com/intent/tweet?url=https://exokeryx.com/exoblog/extracellular-vesicle-isolation-with-demeter-evprep-demonstrates-compatibility-with-mass-spectrometry-proteomic-analysis) [pinterest-p icon ](http://pinterest.com/pin/create/link/?url=https://exokeryx.com/exoblog/extracellular-vesicle-isolation-with-demeter-evprep-demonstrates-compatibility-with-mass-spectrometry-proteomic-analysis) [envelope icon ](mailto:?body=https://exokeryx.com/exoblog/extracellular-vesicle-isolation-with-demeter-evprep-demonstrates-compatibility-with-mass-spectrometry-proteomic-analysis)

Researchers at The Scripps Research Institute Evaluate Demeter EVPrep™ for Compatibility with Mass Spectrometry

3:01

[![Volcano Plot2](https://22732406.fs1.hubspotusercontent-na1.net/hub/22732406/hubfs/Volcano%20Plot2.png?width=1200&length=1200&name=Volcano%20Plot2.png) ](https://exokeryx.com/white-paper-extracellular-vesicle-isolation-with-demeter-evprep-demonstrates-compatibility-with-mass-spectrometry-proteomic-analysis)

Volcano plot showing proteins identified in Demeter EVPrep-isolated EVs from wild type (WT) and Alzheimer’s Disease (AD) organoid cultures.

 

The success of EV research depends on scalable, automated, reproducible isolation methods that consistently produce high recovery of pure EVs. Conventional EV isolation methods miss the mark, forcing a tradeoff between purity and recovery and producing unreliable results. In a recent study, 78% of EV researchers polled indicated that poor EV isolation quality and lack of reproducibility in EV isolation are key obstacles to achieving their research outcomes*.

> *"If the [EV isolation] method is not consistent within and across experiments, it is difficult to distinguish true biological signals from technical variation. This undermines confidence in downstream analysis...and ultimately weakens the reliability of biological conclusions and translational potential." - EV Researcher**

EXOKĒRYX is leveraging the novel application of chip-based technology and microfluidics to unlock the potential of extracellular vesicles and deliver better tools for EV researchers. While conventional methods use a single physical property to isolate EVs, our [Demeter EVPrepTM](https://exokeryx.com/demeter-evprep) isolates EVs based on both size and charge simultaneously, selectively isolating EVs to deliver greater recovery and purity than other techniques. And its fully-automated workflow enables reproducibility not possible with more manual, hands-on methods.

Dr. [Anthony Balistreri](https://www.linkedin.com/in/abalist/) studies Alzheimer's Disease in the [Kelly Lab](https://www.scripps.edu/kelly/) at The Scripps Research Institute. He has experienced the challenges with conventional EV isolation firsthand. In our latest white paper, he and fellow Scripps Research colleague Dr. [Natalie Turner](https://www.linkedin.com/in/natalie-turner-au/) ([Yates Lab](https://www.scripps.edu/yates/index.html)) share the results of a limited technology evaluation study that looked at Demeter EVPrep's ability to:

1. 1. Produce pure EV eluates compatible with mass spectrometry proteomic analysis.
     2. Isolate EVs containing proteins associated with Alzheimer's Disease.

The mass spectrometry proteomic analysis revealed deep proteome coverage in the Demeter EVPrep EV isolates, demonstrating compatibility of Demeter EVPrep with mass spectrometry. Amyloid precursor proteins A4 and Tau were also detected. When A4 is cleaved, it results in amyloid-beta peptides, the pathogenic peptides associated with Alzheimer’s disease. There is a dearth of widely accessible biomarkers for Alzheimer's Disease that enable early, preclinical diagnosis. EV proteomic analysis holds great potential for identifying such a diagnostic tool. Demeter EVPrep, with its demonstrated compatibility with mass spectrometry analysis, isolation of EVs containing proteins associated with Alzheimer's Disease, and hands-off, automated workflow that makes it reproducible by design, may become a key player in helping to identify biomarkers for early Alzheimer's Disease diagnosis.

Read the white paper here: [Extracellular Vesicle Isolation with ](https://exokeryx.com/white-paper-extracellular-vesicle-isolation-with-demeter-evprep-demonstrates-compatibility-with-mass-spectrometry-proteomic-analysis)[Demeter EVPrep Demonstrates Compatibility with Mass Spectrometry Proteomic Analysis](https://exokeryx.com/white-paper-extracellular-vesicle-isolation-with-demeter-evprep-demonstrates-compatibility-with-mass-spectrometry-proteomic-analysis).

*Data on file at EXOKĒRYX.

[Evidence](https://exokeryx.com/exoblog/tag/evidence), [Alzheimer's Disease](https://exokeryx.com/exoblog/tag/alzheimers-disease), [Mass Spectrometry](https://exokeryx.com/exoblog/tag/mass-spectrometry), [Demeter EVPrep](https://exokeryx.com/exoblog/tag/demeter-evprep)

## Related posts

[![Dr. Caitlin McAtee, Exokeryx Field Application Scientist, presenting her abstract on Demeter EVPrep.](https://exokeryx.com/hs-fs/hubfs/IMG_1506.jpg?height=200&name=IMG_1506.jpg) ](https://exokeryx.com/exoblog/vanderbilt-center-for-ev-research-annual-retreat)

[Events](https://exokeryx.com/exoblog/tag/events)

## [Vanderbilt Center for EV Research Annual Retreat](https://exokeryx.com/exoblog/vanderbilt-center-for-ev-research-annual-retreat)

![Picture of Caitlin McAtee](https://exokeryx.com/hs-fs/hubfs/Caitlin%20Headshot.jpg?width=50&name=Caitlin%20Headshot.jpg) [Caitlin McAtee](https://exokeryx.com/exoblog/author/caitlin-mcatee) 

 Oct 14, 2025 3:39:29 PM

[ Read more ](https://exokeryx.com/exoblog/vanderbilt-center-for-ev-research-annual-retreat)

[![Dr. Caitlin McAtee presents Demeter EVPrep and dielectrophoresis for EV isolation to attendees of AAEV's 2025 Annual Meeting.](https://exokeryx.com/hs-fs/hubfs/Image%20(76).jpg?height=200&name=Image%20(76).jpg) ](https://exokeryx.com/exoblog/2025-selectbio-and-aaev)

[Conferences](https://exokeryx.com/exoblog/tag/conferences)

## [Challenges with EV Isolation a Key Topic at Recent EV Conferences](https://exokeryx.com/exoblog/2025-selectbio-and-aaev)

![Picture of Caitlin McAtee](https://exokeryx.com/hs-fs/hubfs/Caitlin%20Headshot.jpg?width=50&name=Caitlin%20Headshot.jpg) [Caitlin McAtee](https://exokeryx.com/exoblog/author/caitlin-mcatee) 

 Dec 8, 2025 4:00:00 PM

[ Read more ](https://exokeryx.com/exoblog/2025-selectbio-and-aaev)

[![Dr. Caitlin McAtee presents Demeter EVPrep and dielectrophoresis for EV isolation to attendees of ISEV's 2nd Symposium on EVs in Nervous Systems.](https://exokeryx.com/hs-fs/hubfs/Image%20%2849%29.jpg?height=200&name=Image%20%2849%29.jpg) ](https://exokeryx.com/exoblog/evins-2025)

[Conferences](https://exokeryx.com/exoblog/tag/conferences)

## [2nd Symposium on Extracellular Vesicles in Nervous Systems](https://exokeryx.com/exoblog/evins-2025)

![Picture of Janet Anna](https://exokeryx.com/hs-fs/hubfs/Janet%20Headshot%20(1).jpg?width=50&name=Janet%20Headshot%20(1).jpg) [Janet Anna](https://exokeryx.com/exoblog/author/janet-anna) 

 Nov 10, 2025 2:13:23 PM

[ Read more ](https://exokeryx.com/exoblog/evins-2025)

```json
{
  "@context" : "https://schema.org",
  "@type" : "BlogPosting",
  "author" : {
    "@type" : "Person",
    "name" : "Janet Anna",
    "url" : "https://exokeryx.com/exoblog/author/janet-anna"
  },
  "dateModified" : "2026-02-16T19:42:46.602Z",
  "datePublished" : "2026-02-16T19:42:46.000Z",
  "headline" : "Researchers at The Scripps Research Institute Evaluate Demeter EVPrep™ for Compatibility with Mass Spectrometry",
  "image" : [ "https://exokeryx.com/hubfs/Volcano%20Plot2.png" ],
  "mainEntityOfPage" : {
    "@id" : "https://exokeryx.com/exoblog/extracellular-vesicle-isolation-with-demeter-evprep-demonstrates-compatibility-with-mass-spectrometry-proteomic-analysis",
    "@type" : "WebPage"
  },
  "publisher" : {
    "@type" : "Organization",
    "logo" : {
      "@type" : "ImageObject",
      "url" : "https://exokeryx.com/hubfs/Exokeryx%20Logo_Full%20Color.png"
    },
    "name" : "EXOKĒRYX"
  }
}
```